Sequence

Analysis Tool	Result
iMutant 3.0	Destabilizing
STRUM	Stabilizing
iStable	Stabilizing
Provean	Neutral
PredictSNP	Neutral
PhD-SNP	Deleterious
PolyPhen-2	Neutral
SIFT	Neutral
FATHMM	Damaging

Structure

Analysis Tool	Result
mCSM	Destabilizing
SDM	Destabilizing
DUET	Destabilizing
DynaMut	Stabilizing
CUPSAT	Destabilizing
ENCoM	Destabilizing
FoldX (Protein Stability)	Slightly Reduces

Aggregation

Analysis Tool	Result
TANGO (Aggregation Tendency)	No effect
WALTZ (Amyloid Propensity)	No effect
LIMBO (Chaperone Binding Tendency)	Increases

Reported Articles

• Nomura T, Yamashita T, Misumi Y, Ueda M, Masuda T, Tasaki M, et al. A case with a novel variant transthyretin A36D presenting cardiac phenotype. Amyloid. 2017;24: 100–100. doi:10.1080/13506129.2017.1287072
• Yamashita T, Ueda M, Saga N, Nanto K, Tasaki M, Masuda T, et al. Hereditary amyloidosis with cardiomyopathy caused by the novel variant transthyretin A36D. Amyloid. 2016;23: 1–2. doi:10.1080/13506129.2016.1202228