Sequence

Analysis Tool	Result
iMutant 3.0	Destabilizing
STRUM	Destabilizing
iStable	Destabilizing
Provean	Neutral
PredictSNP	Neutral
PhD-SNP	Neutral
PolyPhen-2	Deleterious
SIFT	Neutral
FATHMM	Damaging

Structure

Analysis Tool	Result
mCSM	Destabilizing
SDM	Stabilizing
DUET	Destabilizing
DynaMut	Stabilizing
CUPSAT	Stabilizing
ENCoM	Destabilizing
FoldX (Protein Stability)	Slightly Reduces

Aggregation

Analysis Tool	Result
TANGO (Aggregation Tendency)	No effect
WALTZ (Amyloid Propensity)	No effect
LIMBO (Chaperone Binding Tendency)	No effect

Reported Articles

• Klaassen SHC, Lemmink HH, Bijzet J, Glaudemans AWJM, Bos R, Plattel W, et al. Late onset cardiomyopathy as presenting sign of ATTR A45G amyloidosis caused by a novel TTR mutation (p.A65G). Cardiovasc Pathol. 2017;29: 19–22. doi:10.1016/j.carpath.2017.04.002