Sequence

Analysis Tool	Result
iMutant 3.0	Destabilizing
STRUM	Destabilizing
iStable	Destabilizing
Provean	Neutral
PredictSNP	Deleterious
PhD-SNP	Deleterious
PolyPhen-2	Deleterious
SIFT	Deleterious
FATHMM	Damaging

Structure

Analysis Tool	Result
mCSM	Destabilizing
SDM	Destabilizing
DUET	Stabilizing
DynaMut	Destabilizing
CUPSAT	Destabilizing
ENCoM	Destabilizing
FoldX (Protein Stability)	No effect

Aggregation

Analysis Tool	Result
TANGO (Aggregation Tendency)	No effect
WALTZ (Amyloid Propensity)	No effect
LIMBO (Chaperone Binding Tendency)	No effect

Reported Articles

• Togashi S, Watanabe H, Nagasaka T, Shindo K, Shiozawa Z, Maeda S, Tawata M, and Onaya T. (1999). An aggressive familial amyloidotic polyneuropathy caused by a new variant transthyretin Lys 54. Neurology. 53:637-639.
• Miyata M, Sato T, Mizuguchi M, Nakamura T, Ikemizu S, Nabeshima Y, et al. Role of the glutamic acid 54 residue in transthyretin stability and thyroxine binding. Biochemistry. 2010;49: 114–123. doi:10.1021/bi901677z