Sequence

Analysis Tool	Result
iMutant 3.0	Destabilizing
STRUM	Stabilizing
iStable	Destabilizing
Provean	Deleterious
PredictSNP	Neutral
PhD-SNP	Deleterious
PolyPhen-2	Neutral
SIFT	Neutral
FATHMM	Damaging

Structure

Analysis Tool	Result
mCSM	Destabilizing
SDM	Destabilizing
DUET	Destabilizing
DynaMut	Destabilizing
CUPSAT	Destabilizing
ENCoM	Destabilizing
FoldX (Protein Stability)	Severely Reduces

Aggregation

Analysis Tool	Result
TANGO (Aggregation Tendency)	No effect
WALTZ (Amyloid Propensity)	No effect
LIMBO (Chaperone Binding Tendency)	No effect

Reported Articles

• Uemichi T, Uitti RJ, Koeppen AH, Donat JR, Benson MD. Oculoleptomeningeal amyloidosis associated with a new transthyretin variant Ser64. Arch Neurol. 1999;56: 1152–1155. doi:10.1001/archneur.56.9.1152
• Connors LH, Theberge R, Skare J, Amico A, Costello CE, and Skinner M. (1999). Defining Familial Transthyretin Amyloidosis: Current Clinical Testing Approach. The 4th Int’l Symp on FAP and Other TTR Related Disorders. Umeå Sweden, June 1999.