Sequence

Analysis Tool	Result
iMutant 3.0	Destabilizing
STRUM	Destabilizing
iStable	Destabilizing
Provean	Neutral
PredictSNP	Neutral
PhD-SNP	Neutral
PolyPhen-2	Neutral
SIFT	Deleterious
FATHMM	Damaging

Structure

Analysis Tool	Result
mCSM	Destabilizing
SDM	Stabilizing
DUET	Destabilizing
DynaMut	Destabilizing
CUPSAT	Destabilizing
ENCoM	Destabilizing
FoldX (Protein Stability)	No effect

Aggregation

Analysis Tool	Result
TANGO (Aggregation Tendency)	Increases
WALTZ (Amyloid Propensity)	No effect
LIMBO (Chaperone Binding Tendency)	Increases

Reported Articles

• Altland, K (1999). Common molecular characteristics of amyloidogenic TTR mutations. The 4th International Symposium on FAP and Other TTR Related Disorders. Umeå, Sweden, June 1999
• Zhao L, Buxbaum JN, Reixach N. Age-related oxidative modifications of transthyretin modulate its amyloidogenicity. Biochemistry. 2013;52: 1913–1926. doi:10.1021/bi301313b
• Altland K, Benson MD, Costello CE, Ferlini A, Hazenberg BPC, Hund E, et al. Genetic microheterogeneity of human transthyretin detected by IEF. ELECTROPHORESIS. 2007;28: 2053–2064. doi:10.1002/elps.200600840