Sequence

Analysis Tool	Result
iMutant 3.0	Stabilizing
STRUM	Destabilizing
iStable	Stabilizing
Provean	Neutral
PredictSNP	Neutral
PhD-SNP	Neutral
PolyPhen-2	Neutral
SIFT	Neutral
FATHMM	Damaging

Structure

Analysis Tool	Result
mCSM	Destabilizing
SDM	Stabilizing
DUET	Stabilizing
DynaMut	Destabilizing
CUPSAT	Destabilizing
ENCoM	Destabilizing
FoldX (Protein Stability)	No effect

Aggregation

Analysis Tool	Result
TANGO (Aggregation Tendency)	No effect
WALTZ (Amyloid Propensity)	No effect
LIMBO (Chaperone Binding Tendency)	No effect

Reported Articles

• Connors LH, Theberge R, Skare J, Amico A, Costello CE, and Skinner M. (1999). Defining Familial Transthyretin Amyloidosis: Current Clinical Testing Approach. The 4th Int’l Symp on FAP and Other TTR Related Disorders. Umeå Sweden, June 1999.
• Connors LH, Theberge R, Skare J, Costello CE, Falk RH, and Skinner M. (1999). A new transthyretin variant (Ser23Asn) associated with familial amyloidosis in a Portuguese patient. Amyloid: Int J Exp Clin Invest. 6:114-118.
• Theberge R, Connors L, Skinner M, and Costello CE. (1999). Characterization of transthyretin mutants from serum using immunoprecipitation, HPLC/electrospray ionization and matrix-assisted laser desorption/ionization mass spectrometry. Anal Chem. 71:452-459.